A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes
نویسندگان
چکیده
منابع مشابه
Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean
We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.11.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones wer...
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Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.19.4009